# MISSHA Time Revolution Night Repair Probio Ampoule for mature skin

**Lovi Fit: High fit for mature skin.**
Normal Skin Fit: High · Effectiveness: High · Safety: High.

Page: https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin

The Lovi Fit is the same in every country; only the stores and prices differ, and the ones here are for Canada.

Brand: [MISSHA](https://lovi.care/skincare/ca/brands/missha)
Category: [Serums and treatments](https://lovi.care/skincare/ca/best/serums)

This fit is calculated for the concerns frequently associated with mature skin: fine lines, wrinkles, and loss of firmness. For scores based on a full skin profile (12D face scan, goals, preferences), use the Lovi app.

A great choice for mature skin; a few serums fit it even better.

## Why this fit

- This product is highly suitable for your normal skin, as Bifida Lysate Ferment and Niacinamide work synergistically to strengthen your skin barrier and provide essential hydration. Adenosine further supports your goal of reducing wrinkles and fine lines by promoting collagen production.
- The inclusion of Lactobacillus Ferment and Lactobacillus Soybean Extract Ferment offers additional benefits for skin texture and resilience.
- The safety profile is excellent, with no risks of irritation due to the absence of harsh ingredients and the presence of soothing components.

## Fit by skin type

- [Oily skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/oily-skin): Average
- [Dry skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/dry-skin): High
- [Combination skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/combination-skin): Average
- [Sensitive skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/sensitive-skin): High
- [Acne-prone skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/acne-prone-skin): Average
- [Skin with dark spots](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/dark-spots): High
- [Mature skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin): High (this page)
- [Dry sensitive skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/dry-sensitive-skin): High
- [Oily acne-prone skin](https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/oily-acne-prone-skin): Average

## Key ingredients

Bifida Lysate Ferment, Niacinamide, Sodium Hyaluronate, Probio Lactobacillus Complex, Panthenol

## What the research says

- Adenosine-loaded dissolving microneedle patches showed similar or better efficacy on skin wrinkles, dermal density, elasticity, and hydration compared to adenosine cream in 22 female subjects. ([International Journal of Cosmetic Science, 2018](https://pubmed.ncbi.nlm.nih.gov/29574973/))
- Horse oil-spread and adenosine-loaded dissolving microneedle patches significantly improved skin elasticity, hydration, dermal density, and wrinkles compared with adenosine-loaded dissolving microneedle patches in 20 women without causing adverse events. ([Journal of Cosmetic Dermatology, 2019](https://pubmed.ncbi.nlm.nih.gov/30160014/))
- Topical application of a disodium adenosine monophosphate-based formulation reduced wrinkles and hyperpigmentation while improving hydration, barrier function, skin lifting, and epidermal turnover in female participants. ([International Journal of Molecular Sciences, 2026](https://pubmed.ncbi.nlm.nih.gov/42278371/))
- Topical application of 1.0% Lactobacillus ferment from jojoba cake significantly increased skin collagen fluorescence compared to baseline and placebo cream over 56 days in 46 individuals. ([International Journal of Cosmetic Science, 2026](https://pubmed.ncbi.nlm.nih.gov/42698229/))
- Topical dexpanthenol acts as a moisturizer, improves stratum corneum hydration, accelerates wound healing and epidermal regeneration, reduces erythema, and improves symptoms of skin irritation compared to vehicle or no pretreatment. ([American Journal of Clinical Dermatology, 2002](https://pubmed.ncbi.nlm.nih.gov/12113650/))
- Topical application of Bifida Ferment Lysate strengthens the skin barrier function by upregulating barrier and antimicrobial peptide genes, exhibiting strong antioxidant properties, and decreasing inflammatory cytokine secretion in vitro. ([Journal of Cosmetic Dermatology, 2023](https://pubmed.ncbi.nlm.nih.gov/37218728/))
- Topical post-laser application of human adipocyte-derived mesenchymal stem cell media combined with niacinamide significantly improved wrinkle index, melanin index, patient satisfaction, and investigator esthetic improvement scores compared to vehicle cream in patients with aging skin. ([Journal of Cosmetic Dermatology, 2021](https://pubmed.ncbi.nlm.nih.gov/33103316/))
- Topical nicotinamide prevented solar-simulated UV-induced immunosuppression in healthy volunteers, and men experienced immunosuppression at solar-simulated UV doses three times lower than those required for women. ([The Journal of Investigative Dermatology, 2008](https://pubmed.ncbi.nlm.nih.gov/17882270/))
- Topical 5% niacinamide applied twice daily for 12 weeks reduced fine lines and wrinkles, hyperpigmented spots, red blotchiness, and skin sallowness, and improved skin elasticity compared to vehicle control in 50 female white subjects with facial photoaging. ([Dermatologic Surgery, 2005](https://pubmed.ncbi.nlm.nih.gov/16029679/))
- Topical application of 0.1% hyaluronic acid formulations of different molecular weights led to significant improvements in skin hydration and elasticity, with low-molecular-weight formulations also significantly reducing wrinkle depth in seventy-six female subjects compared to a vehicle control. ([Journal of Drugs in Dermatology, 2011](https://pubmed.ncbi.nlm.nih.gov/22052267/))
- Topical application of hyaluronic acid in conjunction with free gingival graft significantly reduced donor site postoperative pain until day 7 compared to normal saline, with no significant differences in graft dimensions or patient satisfaction. ([Clinical Oral Investigations, 2022](https://pubmed.ncbi.nlm.nih.gov/34623507/))
- Treatment with a hyaluronic acid-containing mouth rinse or topical gel led to significant lesion reduction, pain relief, and normalization of lesion color in adult patients with minor recurrent aphthous stomatitis, with the gel formulation showing an earlier healing onset at 3 days. ([BMC Oral Health, 2019](https://pubmed.ncbi.nlm.nih.gov/31311529/))
- Probiotic-derived lotion successfully reduced inflammatory acne lesion counts and erythema index comparably to 2.5% benzoyl peroxide in patients with mild-to-moderate acne vulgaris, while causing fewer side effects. ([Journal of Cosmetic Dermatology, 2022](https://pubmed.ncbi.nlm.nih.gov/35384257/))
- Topical application of Lactobacillus plantarum-GMNL6 enhanced collagen synthesis and SPTSSA gene expression while reducing melanin synthesis, Staphylococcus aureus biofilm, and Cutibacterium acnes proliferation, leading to clinical improvements in skin moisture, color, spots, wrinkles, UV spots, and porphyrins in human volunteers. ([International Journal of Medical Sciences, 2021](https://pubmed.ncbi.nlm.nih.gov/33526970/))
- Lactobacillus plantarum extracellular vesicles modulated extracellular matrix related gene expression in vitro and suppressed wrinkle formation and pigmentation in clinical trials. ([Current Issues in Molecular Biology, 2022](https://pubmed.ncbi.nlm.nih.gov/35723322/))
- Moisturizer containing 5% panthenol, madecassoside, and copper-zinc-manganese decreased adverse reactions and downtime after ablative fractional carbon dioxide laser resurfacing with comparable efficacy to 0.02% triamcinolone acetonide cream in 20 subjects. ([Journal of Cosmetic Dermatology, 2019](https://pubmed.ncbi.nlm.nih.gov/30945430/))
- Topical dexpanthenol formulated in lipophilic vehicles improved stratum corneum hydration and reduced transepidermal water loss compared to vehicle control in humans. ([Arzneimittel-Forschung, 2000](https://pubmed.ncbi.nlm.nih.gov/10965426/))
- Hyaluronic acid, hyaluronic acid plus dexpanthenol, and isotonic saline nasal sprays all significantly improved dry nose symptoms over four weeks in 240 patients, with hyaluronic acid plus dexpanthenol providing superior nasal moisturisation compared to the other two. ([Auris, Nasus, Larynx, 2020](https://pubmed.ncbi.nlm.nih.gov/32067777/))

## What reviewers say

This ampoule is frequently praised for delivering a 'glass skin' effect overnight, making dry and mature skin appear plump, hydrated, and glowing. A very large amount of data suggests that while it's a highly effective and affordable alternative to luxury serums, the high concentration of fermented ingredients may cause breakouts in individuals with fungal acne or sensitivities.

- Liked: Instant Glow (mostly mentioned)
- Liked: Deep Hydration (often mentioned)
- Liked: Lightweight Texture (often mentioned)
- Liked: Texture Improvement (sometimes noted)
- Liked: Value for Money (sometimes noted)
- Disliked: Breakouts & Reactions (sometimes noted)
- Disliked: Fragrance & Oils (sometimes noted)
- Disliked: Dropper Issues (rarely noted)
- Disliked: Ineffective for Some (rarely noted)
- Disliked: Sticky Residue (rarely noted)

## How to apply

- Use this serum in your PM routine. Start by applying it 3 times a week, and work up to daily use if your skin likes it.
- Apply this serum after your toner and before your moisturizer.
- Gently press 2-3 drops of the serum into your skin and let it absorb for a minute.
- You can apply the serum on dry skin for the best results against fine lines.
- Always do a patch test before your first use and avoid the sensitive eye area.
- Video: https://storage.pora.ai/c879dda8655a61c19d3d8c9ebad6b06d.mp4

## Questions and answers

### What makes MISSHA Time Revolution Night Repair Probio Ampoule a great fit for mature skin?

This product is a great fit for mature skin because Bifida Lysate Ferment and Niacinamide help strengthen your skin barrier while delivering essential moisture. Adenosine also supports your goal of reducing fine lines and wrinkles, and the formula is very unlikely to cause irritation.
Sources: [International Journal of Cosmetic Science, 2018](https://pubmed.ncbi.nlm.nih.gov/29574973/), [International Journal of Molecular Sciences, 2026](https://pubmed.ncbi.nlm.nih.gov/42278371/)

### Which ingredients make this serum a good fit for mature skin?

Bifida Lysate Ferment, Niacinamide, and Sodium Hyaluronate make this a great fit by strengthening your skin barrier and keeping dry areas hydrated. Adenosine, Panthenol, and the probiotic ferments help smooth out fine lines and improve overall skin texture with virtually no risk of irritation.
Sources: [International Journal of Cosmetic Science, 2018](https://pubmed.ncbi.nlm.nih.gov/29574973/), [International Journal of Cosmetic Science, 2026](https://pubmed.ncbi.nlm.nih.gov/42698229/)

### What does Bifida Ferment Lysate do for mature skin?

Bifida Ferment Lysate helps strengthen the skin barrier and calm irritation, which keeps mature skin feeling soft and comfortable. It also provides antioxidant support that may protect against everyday stress, though products for mature skin also need firming and smoothing ingredients like peptides and retinol.
Sources: [Journal of Cosmetic Dermatology, 2023](https://pubmed.ncbi.nlm.nih.gov/37218728/), [Experimental Dermatology, 2010](https://pubmed.ncbi.nlm.nih.gov/19624730/)

### What does Niacinamide do for mature skin?

Niacinamide helps mature skin by smoothing out fine lines and wrinkles, reducing dark spots, and improving overall skin elasticity. It is also very well tolerated and supports a strong skin barrier to keep skin looking healthy.
Sources: [Antioxidants, 2021](https://pubmed.ncbi.nlm.nih.gov/34439563/), [Journal of Cosmetic Dermatology, 2004](https://pubmed.ncbi.nlm.nih.gov/17147561/)

### Where does this serum go in a skincare routine for mature skin?

This treatment goes in your evening routine, after cleansing and toning and before moisturizing, for simplicity and extra safety around sun exposure.

### Can I use this serum with retinol, and in what order?

You can use this serum with retinol by applying the serum first in your evening routine, allowing it to hydrate and strengthen your skin barrier before your retinol step. This product is very well suited for your skin, and the combination is unlikely to cause irritation because it contains soothing components and lacks harsh ingredients.
Sources: [International Journal of Cosmetic Science, 2018](https://pubmed.ncbi.nlm.nih.gov/29574973/), [International Journal of Cosmetic Science, 2026](https://pubmed.ncbi.nlm.nih.gov/42698229/)

### How often should I use this serum?

This serum fits your mature skin very well and is used in the evening routine. It has an excellent safety profile with soothing ingredients, so it is unlikely to cause irritation when used daily.
Sources: [Clinical, Cosmetic and Investigational Dermatology, 2023](https://pubmed.ncbi.nlm.nih.gov/36798538/), [Facial Plastic Surgery, 2014](https://pubmed.ncbi.nlm.nih.gov/24488631/)

### Can this serum clog pores, cause breakouts or purging for mature skin?

This treatment is unlikely to cause irritation or breakouts on mature skin thanks to its very safe formula, though some shoppers do report occasional reactions. It helps keep your skin smooth and hydrated while smoothing out fine lines.
Sources: [International Journal of Cosmetic Science, 2018](https://pubmed.ncbi.nlm.nih.gov/29574973/), [International Journal of Cosmetic Science, 2026](https://pubmed.ncbi.nlm.nih.gov/42698229/)

### Is this serum fragrance-free and alcohol-free?

Yes, it has no fragrance and no drying alcohol.

## Alternatives for mature skin

- [Dr.Althea Vitamin C Boosting Serum](https://lovi.care/skincare/ca/p/dr-althea/vitamin-c-boosting-serum/for/mature-skin) — High fit — CA$27.99 at Amazon
- [The Ordinary Matrixyl 10% + HA](https://lovi.care/skincare/ca/p/the-ordinary/matrixyl-10-ha/for/mature-skin) — High fit — CA$14.40 at Amazon
- [Skin1004 Probio-Cica Intensive Ampoule](https://lovi.care/skincare/ca/p/skin1004/probio-cica-intensive-ampoule/for/mature-skin) — High fit — CA$24.44 at Amazon

## Pairs well with

- Cleanser: [Fresh Soy Face Cleanser](https://lovi.care/skincare/ca/p/fresh/soy-face-cleanser/for/mature-skin) — Superior fit — CA$44.95 at Amazon
- Hydrating Toner: [COSRX Propolis Synergy Toner](https://lovi.care/skincare/ca/p/cosrx/full-fit-propolis-synergy-toner/for/mature-skin) — Superior fit — CA$31.98 at Amazon
- Moisturizer: [Obagi Hydrate Luxe Moisture-Rich Cream](https://lovi.care/skincare/ca/p/obagi/hydrate-luxe/for/mature-skin) — Superior fit — CA$103.73 at Amazon

## Where to buy

- Amazon.ca: CA$45.59 (https://www.amazon.ca/dp/B00ATLVNIS?tag=lovi-care-web-product01-20&linkCode=osi&th=1&psc=1)
Prices checked 6 Nov 2026.

## Ingredients

Bifida Lysate Ferment, Butylene Glycol, Glycerin, Propanediol, Betaine, Water, Niacinamide, Pentylene Glycol, 1,2-Hexanediol, Diethoxyethyl Succinate, Chondrus Crispus Extract, Squalane, Panthenol, Polyglyceryl-10 Laurate, Saccharum Officinarum Extract, Hydrogenated Lecithin, Carbomer, Tromethamine, Xanthan Gum, Polyquaternium-51, Ethylhexylglycerin, Adenosine, Dextrin, Theobroma Cacao Extract, Beta Vulgaris Root Extract, Brassica Oleracea Capitata Leaf Extract, Daucus Carota Sativa Root Extract Carrot Root Extract, Solanum Melongena Fruit Extract, Blueberry Fruit Extract, Bergamot Oil Fruit, Disodium Edta, Sodium Hyaluronate, Lactobacillus Soybean Extract Ferment, Leuconostoc Radish Root Ferment Filtrate, Bifida Ferment Filtrate, Lactobacillus Ferment, Lactobacillus Lysate Ferment, Lactobacillus Acerola Cherry Ferment, Lactobacillus Punica Granatum Extract Fruit Ferment, Pelargonium Graveolens Oil Flower, Helianthus Annuus Seed Oil Sunflower Seed Oil, Citrus Aurantium Dulcis Peel Oil, Lavandula Angustifolia Oil Lavender Oil, Lupinus Albus Seed Extract, Aspergillus Ferment, Swiftlet Nest Extract, Anthemis Nobilis Oil Flower, Sandalwood Oil, Tocopherol, Ceramide Np, Cholesterol
## About the Lovi Fit

Lovi: Unbiased, science-based skincare AI trained on real user outcomes.

Lovi calculates every fit score from the full ingredient list with brand-independent rules its dermatologists and cosmetic chemists build and review, and personalizes every pick with AI trained on the real results of millions of users. Lovi trains its fit score models on a proprietary dataset built from more than 10,000 expert-labeled products and more than 100,000 simulated examples, following an internally consistent methodology presented at Cosmoprof, AMWC, and Skin of Color Update. Levels from best to worst: Superior, High, Average, Low, Caution, Warning.

Behind this fit score: Nadia Kapleva, MS (Co-founder, Medical Director); Alexey Shagraev, PhD (Co-founder, CEO and CTO); Dr. Sofía Perea Pérez, MD, MSc (Medical Board); Aishani Shah, MD (Medical Board); Asya Zubkova, MS (Medical Board); Hanna Kurets, MD (Medical Board, Medical Advisor); Elina Nazarenko, MD (Medical Board); Victoria Derkova, MS (Medical Board).

Page built Nov 8, 2026.

More from Lovi: https://lovi.care/skincare/llms.txt

## This page in other countries

- United States: https://lovi.care/skincare/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- United Kingdom: https://lovi.care/skincare/uk/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Ireland: https://lovi.care/skincare/ie/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Germany: https://lovi.care/skincare/de/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- France: https://lovi.care/skincare/fr/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Italy: https://lovi.care/skincare/it/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Netherlands: https://lovi.care/skincare/nl/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Spain: https://lovi.care/skincare/es/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Canada: https://lovi.care/skincare/ca/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin (this page)
- Australia: https://lovi.care/skincare/au/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Singapore: https://lovi.care/skincare/sg/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- Saudi Arabia: https://lovi.care/skincare/sa/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin
- United Arab Emirates: https://lovi.care/skincare/ae/p/missha/time-revolution-night-repair-probio-ampoule/for/mature-skin